SOP for Use of Surfactants in Nanoparticle Formulations

Standard Operating Procedure for Surfactant Use in Nanoparticle Formulations

1) Purpose

This SOP provides guidelines for the appropriate selection, handling, and use of surfactants in nanoparticle formulations to ensure proper stabilization of particles and consistency in product quality.

2) Scope

This SOP applies to all laboratory staff involved in the preparation of nanoparticle formulations requiring surfactants for stabilization or formulation purposes.

3) Responsibilities

Operators: Responsible for the proper selection and use of surfactants in nanoparticle formulations.
QA: Ensures surfactant usage complies with the formulation protocol and meets quality standards.

4) Procedure

4.1 Selection of Surfactants

4.1.1 Criteria for Surfactant Selection

4.1.1.1 Select surfactants based on their compatibility with the nanoparticle material, type of formulation, and intended application (e.g., Tween 80, SDS).
4.1.1.2 Review the Material Safety Data Sheets (MSDS) for each surfactant to ensure safe handling and usage.

4.2 Surfactant Handling

4.2.1 Weighing and Dispensing

4.2.1.1 Accurately weigh the required amount of surfactant using a calibrated balance, as per the formulation guidelines.
4.2.1.2 Dispense the surfactant into clean, labeled containers for use in the formulation.

4.2.2 Solubilization of Surfactants

4.2.2.1 Dissolve the surfactant in water or the relevant solvent under gentle stirring to ensure proper solubilization before adding it to the nanoparticle formulation.
4.2.2.2 Ensure the temperature does not exceed the stability

limits of the surfactant during solubilization.

4.3 Addition of Surfactants to Nanoparticle Formulations

4.3.1 Incorporation of Surfactants

4.3.1.1 Add the dissolved surfactant slowly into the nanoparticle formulation under continuous stirring to avoid clumping or aggregation.
4.3.1.2 Continue stirring for at least 15–30 minutes to ensure uniform distribution of the surfactant throughout the formulation.

4.4 Safety and Handling

4.4.1 Safety Guidelines

4.4.1.1 Wear appropriate PPE, including gloves, goggles, and lab coats, when handling surfactants.
4.4.1.2 Work in a fume hood or well-ventilated area if the surfactant is volatile or hazardous.

5) Abbreviations, if any

SDS: Sodium Dodecyl Sulfate
PPE: Personal Protective Equipment

6) Documents, if any

Surfactant Usage Logbook

7) References, if any

MSDS for surfactants

8) SOP Version

Version 1.0

Annexure

Surfactant Usage Logbook Template


  
    Date
    Surfactant Name
    Batch Number
    Amount Used
    Operator Initials
    QA Initials
  
  
    DD/MM/YYYY
    Surfactant Name
    Batch Number
    Amount in grams
    Operator Name
    QA Name

Date	Surfactant Name	Batch Number	Amount Used	Operator Initials	QA Initials
DD/MM/YYYY	Surfactant Name	Batch Number	Amount in grams	Operator Name	QA Name