SOP for Preparation of Magnetic Nanoparticles for Biomedical Applications

Standard Operating Procedure for Preparation of Magnetic Nanoparticles for Biomedical Applications

1) Purpose

This SOP outlines the procedure for the preparation of magnetic nanoparticles used in biomedical applications, such as targeted drug delivery, magnetic resonance imaging (MRI), and hyperthermia treatment.

2) Scope

This SOP applies to laboratory personnel involved in the preparation and characterization of magnetic nanoparticles for biomedical use.

3) Responsibilities

Operators: Responsible for preparing magnetic nanoparticles following the outlined protocol.
QA: Ensures the prepared nanoparticles meet the required specifications for size, magnetic properties, and biocompatibility.

4) Procedure

4.1 Preparation of Magnetic Nanoparticles

4.1.1 Material Selection

4.1.1.1 Select suitable magnetic materials, such as iron oxide (Fe₃O₄ or Fe₂O₃), for nanoparticle synthesis.
4.1.1.2 Ensure that all materials are of high purity and suitable for biomedical applications.

4.2 Synthesis of Magnetic Nanoparticles

4.2.1 Co-Precipitation Method

4.2.1.1 Dissolve ferric chloride (FeCl₃) and ferrous chloride (FeCl₂) in water at a 2:1 molar ratio under nitrogen atmosphere.
4.2.1.2 Add ammonium hydroxide (NH₄OH) dropwise under constant stirring to precipitate magnetic nanoparticles.
4.2.1.3 Continue stirring for 30 minutes to ensure uniform particle formation.

4.3 Surface Functionalization

4.3.1 Coating with Biocompatible Materials

4.3.1.1 Coat the magnetic nanoparticles with a biocompatible material such as PEG, dextran, or silica to enhance their stability and compatibility with biological systems.

4.3.2 Ligand Attachment

4.3.2.1 Attach

specific targeting ligands (e.g., antibodies, peptides) to the surface of the nanoparticles for targeted biomedical applications.

4.4 Particle Size and Magnetic Property Characterization

4.4.1 Dynamic Light Scattering (DLS)

4.4.1.1 Measure the size and distribution of magnetic nanoparticles using DLS.

4.4.2 Magnetic Measurements

4.4.2.1 Use a vibrating sample magnetometer (VSM) or similar equipment to measure the magnetic properties (e.g., saturation magnetization, coercivity) of the nanoparticles.

4.5 Storage

4.5.1 Storage Conditions

4.5.1.1 Store the magnetic nanoparticles in sealed containers at 4°C or room temperature, depending on their stability and intended application.

5) Abbreviations, if any

DLS: Dynamic Light Scattering
VSM: Vibrating Sample Magnetometer
PEG: Polyethylene Glycol

6) Documents, if any

Magnetic Nanoparticle Preparation Logbook

7) References, if any

Relevant literature on magnetic nanoparticles for biomedical applications

8) SOP Version

Version 1.0

Annexure

Magnetic Nanoparticle Preparation Logbook Template


  
    Date
    Batch Number
    Material Used
    Particle Size
    Magnetic Properties
    Operator Initials
    QA Initials
  
  
    DD/MM/YYYY
    Batch Number
    Material (e.g., Fe₃O₄)
    Size in nm
    Magnetic Properties
    Operator Name
    QA Name

Date	Batch Number	Material Used	Particle Size	Magnetic Properties	Operator Initials	QA Initials
DD/MM/YYYY	Batch Number	Material (e.g., Fe₃O₄)	Size in nm	Magnetic Properties	Operator Name	QA Name