SOP for Preparation of Metal Nanoparticles

Standard Operating Procedure for Preparation of Metal Nanoparticles

1) Purpose

This SOP outlines the process for the synthesis of metal nanoparticles, which are widely used in drug delivery, catalysis, and diagnostic applications. The purpose is to ensure consistency and control in the preparation of metal nanoparticles.

2) Scope

This SOP applies to laboratory personnel responsible for the preparation and characterization of metal nanoparticles for various applications.

3) Responsibilities

Operators: Responsible for accurately preparing metal nanoparticles following this SOP.
QA: Ensures the nanoparticles meet the required size and quality specifications.

4) Procedure

4.1 Preparation of Metal Salt Solution

4.1.1 Metal Salt Selection

4.1.1.1 Select the appropriate metal salt (e.g., silver nitrate, gold chloride) based on the type of nanoparticles to be synthesized.

4.1.2 Solution Preparation

4.1.2.1 Dissolve the metal salt in distilled water or a suitable solvent under constant stirring.

4.2 Nanoparticle Formation

4.2.1 Reduction of Metal Ions

4.2.1.1 Add a reducing agent (e.g., sodium borohydride, citrate) to the metal salt solution while stirring to initiate the reduction process.
4.2.1.2 Continue stirring for 30 minutes to ensure complete reduction of the metal ions.

4.2.2 Particle Stabilization

4.2.2.1 Add a stabilizing agent (e.g., PVP or PEG) to prevent nanoparticle aggregation and enhance stability.

4.3 Washing and Purification

4.3.1 Centrifugation

4.3.1.1 Centrifuge the nanoparticle solution at 10,000 g to collect the particles and discard the supernatant.
4.3.1.2 Wash the particles with distilled water and repeat centrifugation as needed.

4.4 Characterization of Nanoparticles

4.4.1 Particle Size Measurement

4.4.1.1 Use dynamic light scattering (DLS) or electron microscopy to measure the particle size and confirm that the nanoparticles meet the desired specifications.

4.5 Storage of Nanoparticles

4.5.1 Storage Conditions

4.5.1.1 Store the metal nanoparticles in sealed containers at room temperature or in refrigeration, depending on the stability requirements.

5) Abbreviations, if any

DLS: Dynamic Light Scattering
PEG: Polyethylene Glycol
PVP: Polyvinylpyrrolidone

6) Documents, if any

Metal Nanoparticle Synthesis Logbook

7) References, if any

Material Safety Data Sheets (MSDS) for metal salts and reagents

8) SOP Version

Version 1.0

Annexure

Metal Nanoparticle Synthesis Logbook Template


  
    Date
    Batch Number
    Metal Type
    Reduction Method
    Particle Size
    Operator Initials
    QA Initials
  
  
    DD/MM/YYYY
    Batch Number
    Metal (e.g., Gold, Silver)
    Reduction Method Used (e.g., Chemical, Photochemical)
    Size in nm
    Operator Name
    QA Name

Date	Batch Number	Metal Type	Reduction Method	Particle Size	Operator Initials	QA Initials
DD/MM/YYYY	Batch Number	Metal (e.g., Gold, Silver)	Reduction Method Used (e.g., Chemical, Photochemical)	Size in nm	Operator Name	QA Name