Standard Operating Procedure for Preparation of Metal Nanoparticles

1) Purpose

This SOP outlines the process for the synthesis of metal nanoparticles, which are widely used in drug delivery, catalysis, and diagnostic applications. The purpose is to ensure consistency and control in the preparation of metal nanoparticles.

2) Scope

This SOP applies to laboratory personnel responsible for the preparation and characterization of metal nanoparticles for various applications.

3) Responsibilities

• Operators: Responsible for accurately preparing metal nanoparticles following this SOP.
• QA: Ensures the nanoparticles meet the required size and quality specifications.

4) Procedure

4.1 Preparation of Metal Salt Solution

4.1.1 Metal Salt Selection

• 4.1.1.1 Select the appropriate metal salt (e.g., silver nitrate, gold chloride) based on the type of nanoparticles to be synthesized.

4.1.2 Solution Preparation

• 4.1.2.1 Dissolve the metal salt in distilled water or a suitable solvent under constant stirring.

4.2 Nanoparticle Formation

4.2.1 Reduction of Metal Ions

• 4.2.1.1 Add a reducing agent (e.g., sodium borohydride, citrate) to the metal salt solution while stirring to initiate the reduction process.
• 4.2.1.2 Continue stirring for 30 minutes to ensure complete reduction of the metal ions.

4.2.2 Particle Stabilization

• 4.2.2.1 Add a stabilizing agent (e.g., PVP or PEG) to prevent nanoparticle aggregation and enhance stability.

4.3 Washing and Purification

4.3.1 Centrifugation

• 4.3.1.1 Centrifuge the nanoparticle solution at 10,000 g to collect