SOP for Preparation of Oil-in-Water (O/W) Emulsions Preparation of Oil-in-Water (O/W) Emulsions 1) Purpose The purpose of this SOP is to outline the procedure for preparing oil-in-water (O/W) emulsions. In O/W emulsions, oil droplets are dispersed in an aqueous continuous phase, stabilized by emulsifying agents. These emulsions are widely used in pharmaceutical, cosmetic, and food…

SOP for Use of Surfactants in Emulsion Formulations Use of Surfactants in Emulsion Formulations 1) Purpose The purpose of this SOP is to outline the procedure for the selection and use of surfactants in emulsion formulations. Surfactants play a critical role in stabilizing emulsions by reducing the surface tension between two immiscible phases, such as…

SOP for Formulation of Cationic Liposomes for DNA Delivery Formulation of Cationic Liposomes for DNA Delivery 1) Purpose The purpose of this SOP is to describe the procedure for the formulation of cationic liposomes for DNA delivery. Cationic liposomes are used for delivering genetic material such as plasmid DNA or siRNA into cells due to…

SOP for Preparation of Immunoliposomes for Targeted Drug Delivery Preparation of Immunoliposomes for Targeted Drug Delivery 1) Purpose The purpose of this SOP is to describe the procedure for the preparation of immunoliposomes. Immunoliposomes are liposomes that are surface-modified with antibodies or antibody fragments for targeted drug delivery. These liposomes bind specifically to target cells,…

SOP for Incorporation of Targeting Ligands in Liposome Formulations Incorporation of Targeting Ligands in Liposome Formulations 1) Purpose The purpose of this SOP is to describe the procedure for incorporating targeting ligands into liposome formulations. Targeting ligands, such as antibodies, peptides, or small molecules, are added to the surface of liposomes to enable active targeting…

SOP for Preparation of Sterically Stabilized Liposomes Preparation of Sterically Stabilized Liposomes 1) Purpose The purpose of this SOP is to describe the procedure for preparing sterically stabilized liposomes (also known as stealth liposomes). Sterically stabilized liposomes are coated with polymers such as polyethylene glycol (PEG) to extend their circulation time in the bloodstream, reduce…

SOP for Formulating pH-Sensitive Liposomes Formulating pH-Sensitive Liposomes 1) Purpose The purpose of this SOP is to describe the procedure for preparing pH-sensitive liposomes. These liposomes are designed to release their encapsulated contents in response to specific pH changes, making them ideal for targeted drug delivery, particularly in acidic environments such as tumor sites or…

SOP for Preparation of Liposomes for Gene Delivery Preparation of Liposomes for Gene Delivery 1) Purpose The purpose of this SOP is to outline the procedure for preparing liposomes specifically designed for gene delivery applications. Liposomes are effective vehicles for delivering genetic material, such as DNA or RNA, into cells. This SOP will ensure proper…

SOP for Preparation of Unilamellar Vesicles (ULVs) Preparation of Unilamellar Vesicles (ULVs) 1) Purpose The purpose of this SOP is to describe the step-by-step procedure for the preparation of unilamellar vesicles (ULVs). ULVs are liposomes with a single lipid bilayer that encapsulate aqueous and lipid-soluble compounds for drug delivery. This SOP outlines the procedure for…

SOP for Preparation of Multilamellar Vesicles (MLVs) Preparation of Multilamellar Vesicles (MLVs) 1) Purpose This SOP outlines the procedure for the preparation of multilamellar vesicles (MLVs) in liposome formulations. MLVs consist of multiple lipid bilayers and are commonly used in drug delivery systems, as they can encapsulate both hydrophilic and lipophilic drugs. This method is…